Journal: Frontiers in Immunology
Article Title: Identifying specific TLS-associated genes as potential biomarkers for predicting prognosis and evaluating the efficacy of immunotherapy in soft tissue sarcoma
doi: 10.3389/fimmu.2024.1372692
Figure Lengend Snippet: Validation of the function of TNFSF14 by in vitro experiments. (A) TNFSF14 overexpressed was confirmed by western blot assays in SW872 and HT1080 cells. (B, C) CCK-8 assays showed reduced cell proliferation in TNFSF14 overexpressed cells. (D–G) Cell migration and invasion of sarcoma cells were determined by Transwell assays. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, ns, no significance.
Article Snippet: In this experiment, the human liposarcoma cell line SW872 (CL-0685B) and the human fibrosarcoma cell line HT1080 (CL-0117) were purchased from Procell Biotech (Wuhan, China), and passed STR identification and mycoplasma contamination testing.
Techniques: Biomarker Discovery, In Vitro, Western Blot, CCK-8 Assay, Migration